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SRX4714930: Whole genome sequencing of Escherichia coli O111:H8 strain RM9975
2 PACBIO_SMRT (PacBio RS II) runs: 326,964 spots, 1.5G bases, 5.2Gb downloads

Design: Single Molecule, Real-Time (SMRT) sequencing was performed on the Pacific Biosciences (PacBio) RSII sequencing platform using 20-kb SMRTbell libraries, with the P6/C4 sequencing chemistry and 360-min data collection protocols. The SMRTbell libraries were prepared from 10 mg of bacterial genomic DNA, using the standard protocol from Pacific Biosciences, as described in the Procedure-Checklist-20-kb-Template-Preparation-Using-BluePippin-Size-Selection procedure, and processed for sequencing as recommended by the supplier.
Submitted by: United State Department of Agriculture
Study: Escherichia coli O111 Genome sequencing and assembly
show Abstracthide Abstract
The rise in foodborne-related outbreaks of non O157 shiga-toxin producing E. coli (STECs) emphasizes the importance of developing improved methods to rapidly detect and characterize STECs. O111 is a major serovars of STEC associated with foodborne infection and, in some cases, severe illness comparable to illness caused by E. coli O157:H7. Sequencing of STECs from the agriculturally important region of the California central coast will address the diversity of genomic content and physiology of STECs compared to E. coli O157.
Sample: Pathogen: Escherichia coli O111 str. RM9975 from American Crow
SAMN08823043 • SRS3771714 • All experiments • All runs
Library:
Name: RM9975PB1
Instrument: PacBio RS II
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 2 runs, 326,964 spots, 1.5G bases, 5.2Gb
Run# of Spots# of BasesSizePublished
SRR7876071163,482775.3M2.7Gb2018-09-21
SRR7883104163,482760M2.6Gb2018-09-20

ID:
6382749

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